Dr. Tim's Aquatics One & Only Live Nitrifying Bacteria for Freshwater Aquariums

By Dr. Tim's Aquatics
Rated 2.7568 out of 5 stars


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About This Item


  • Prevents and eliminates new tank syndrome by instantly working to establish a biofilter in newly set up aquariums with 100% natural living nitrifiers
  • Controls ammonia and nitrite levels instantly by removing toxic ammonia and nitrite naturally in both freshwater and saltwater aquariums
  • No sulfide or other offensive odors so you don’t have worry about overusing
  • Once added to the tank, simply add fish right away!
  • Shake well before using and keep refrigerated for longest shelf life

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Play Video: Learn More About Dr. Tim's Aquatics From Our Team of Experts
Learn More About Dr. Tim's Aquatics From Our Team of Experts

Special Note
  • 2-oz bottle treats up to 30 gallons.
  • 4-oz bottle treats up to 60 gallons.
  • 8-oz bottle treats up to 120 gallons.

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This Item – Dr. Tim's Aquatics One & Only Live Nitrifying Bacteria for Freshwater Aquariums, 4-oz bottle
Rated 2.7568 out of 5 stars
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Top Ingredients100% Live Nitrifying Bacteria for aquaria
Fish TypeBetta, Bottom Feeder, Cichlid, Discus, Goldfish, Tropical FreshwaterTropical FreshwaterTropical FreshwaterBetta, Bottom Feeder, Cichlid, Discus, Goldfish, Tropical FreshwaterBetta, Bottom Feeder, Cichlid, Discus, Goldfish, Tropical Freshwater
Aquarium TypeFreshwaterFreshwaterFreshwaterFreshwaterFreshwater
Health FeatureImmune Support

Questions & Answers

3 Customer Questions

can I use Dr. Tim's one and only freshwater for water changes?
Answer by ChewyJan 17, 2019
This live nitrifying bacteria is designed to be used during water changes, when setting up a new tank, or after a disease treatment.

is their copper in this
Answer by ChewySep 12, 2018
This product does not contain copper. This treatment contains 100% Live Nitrifying Bacteria for aquaria.

how do you know if the bacteria is dead
Answer by ChewyMar 09, 2021
This product contains live species of ammonia- and nitrite-oxidizing bacteria that prefer to live in the fish tank environment.

ReviewsWrite a Review

Rated 2.7568 out of 5 stars
37 Ratings
Would recommend to a friend

36 Customer Reviews

Rated 5 out of 5 stars
By Buck on Jul 14, 2023

Works Great

I waited 2 and half weeks for my tank to cycle without adding beneficial bacteria. I added Dr. Tim's One and Only and had no ammonia in 24 hours and no nitrite within 72 hours. My tank had cycled within 72 hours of adding this product. I highly recommend it for new aquariums.

Rated 1 out of 5 stars
By Ogre on Dec 15, 2022

Not performing as expected

After 1 week and multiple additions of bacteria have seen no ammonia conversion. To nitrites and nitrates. No I have to spend more money on a different brand to try and get things rolling.

Rated 1 out of 5 stars
By Jonathan42896 on Apr 22, 2022

Useless product

I paired this with their bottle of ammonia and followed directions specifically. I've done fishless cycles before and never had this much of a problem while spending this much extra money.

Rated 5 out of 5 stars
By Dobermom on Aug 1, 2021

New Tank Syndrome Solution

So I dont leave many reviews for products I've bought. Let me tell you, my 60 gallon new aquarium was milky white. Every aquatic enthusiast said to wait and do nothing. So after losing a favorite Oranda, I had to try something. Dr. Tims to the rescue. In 48 hours, it completely cleared, ammonia is 0. Fish are happy and active. Ive added pic to prove it wirks!

Rated 1 out of 5 stars
By Stacy on Jul 21, 2021

No Effect on Tank

Unfortunately there was no change in water parameters after using this product. I doubled the amount needed, using the entire 120mL on a 55 gallon tank. Ammonia did not budge from prior test after a full 24 hours. Still no signs of nitrite or nitrate. It was cooler when this shipped too so I was hoping heat wouldn't impact the effectiveness.

Rated 3 out of 5 stars
By jimbo on Oct 7, 2021

Not sold on this

First time using this. I use to use Stability and had better results. Although many swear by this i will not.

Rated 1 out of 5 stars
By FishMom on Apr 2, 2021

Didn't work at all

All the fish ppl said this was sooo much better. I poured the whole bottle, that should treat 60 gallons, into a 10 gallon tank and nothing happened for 9 days. I put the 40 drops of ammonia(4 drops per gallon as instructed) and it brought the ammonia up to 4ppm for all 9 days, No nitrites and no nitrates. I ended up draining the tank and starting over with Fluval, which I know works in 3 days. That was last night and I already have nitrates with Fluval.

Rated 1 out of 5 stars
By Scout on Mar 14, 2021

Didn’t work- disappointed

I tried doing the fast track tank cycle following instructions to the T and the ammonia is the only thing that worked. I poured the entire bottle in my 40 gallon tank and in the filter. I can’t get the ammonia back down and it’s still showing 0 nitrites with PH of 7.6 after almost 2 weeks. I haven’t been able to even add the extra dose of ammonia in to help kick off the bacteria so I ended up doing a water change and adding other beneficial bacteria & now my tank of finally starting to cycle. I’m so bummed after all the good reviews that it didn’t work with paying all of this money for “the best bacteria” and regular bacteria that’s much cheaper is what’s working

Rated 5 out of 5 stars
By Ryan on Oct 8, 2020

Worked perfectly

My 60 Gallon aquarium wasn’t cycling fast enough so I decided to add this and the ammonia at the same time my ammonia spiked the next day then right after the nitrite spiked now nitrate is spiking so basically I had tried to cycle normally with rotting food and other thing but it still was 1 month later and nothing had really spiked so if you want to cycle a tank in like 2 weeks use this stuff.

Rated 1 out of 5 stars
By Goople80 on Dec 7, 2020

Expired over a year

Ordered from chewy. Best by date is nov 2019. It is dec 2020. Not sure if it’s effective. According to dr Tim the bacteria lives up to a year.